Job ID12248LocationCase Main CampusFull/Part TimeFull-TimeRegular/TemporaryRegularJob DescriptionPOSITION OBJECTIVEWorking under general supervision, the Research Assistant 2 will plan, coordinate and carry out complex research assignments of a non-routine nature concerning the neural control of breathing. These studies include, but are not limited to, performing animal surgery, acquiring various physiological signals related to respiration, analyzing these collected data, conducting biochemical assays, and utilizing new techniques to further the goals of the research program. This position supports and contributes to the Case Western Reserve University School of Medicine's mission to "improve global health by linking research to populations in a superb educational environment." This position will work with animals.ESSENTIAL FUNCTIONSWith general supervision, coordinate, plan and perform laboratory research activities designed to study the neural circuits that control breathing in the presence of systemic inflammation (acute lung injury, peritonitis) and other neurologic impairments in a variety of rodent models (e.g., rats, guinea pigs and mice - including transgenic strains). Experiments in animals include transcardial perfusion, harvesting brain and peripheral tissues, survival and stereotaxic surgery in rodents. (35%)Analyze rodent brain tissue from normal and diseased animals: localize cytokines, chemokines, neurotransmitters and -receptors using in situ hybridization, RNAScope, immunohistochemistry or quantify using assays such as ELISA and Luminex. (20%)Breed rodents, including transgenic strains. (10%)Collect, analyze, and interpret physiologic data (e.g., respiratory frequency, respiratory pattern collected via plethysmography) from healthy and sick animals; run statistical analysis to determine differences between conditions; prepare figures for presentation and publication. (10%)Participate in manuscript and grant writing. Provide data to the principal investigator for sponsor progress reports, manuscripts, grant, and pilot applications. May co-author research manuscripts. (10%)Train new staff, students, clinical fellows rotating through the lab and other full-time laboratory personnel in established experimental techniques, animal models of disease, animal care, and laboratory maintenance; supervise in their daily experimental assignments. (10%)NONESSENTIAL FUNCTIONSMonitor lab inventory, assist in ordering lab supplies and animals, keep lab orderly and organized. (4%)Perform other duties as assigned. (1%)CONTACTSDepartment: Daily contact with supervisor to discuss research and maintain workflow. Daily contact with other lab personnel to coordinate laboratory priorities. Frequent contact with faculty and staff of the Department of Medicine, Division of Pulmonary, Critical Care and Sleep Medicine to discuss experimental results and upcoming plans.University: Occasional contact with other departments to share information. Frequent contact with faculty and staff in other departments to facilitate new and ongoing collaborations. Frequent contact with university personnel to order supplies, order animals, and complete any university required paperwork pertaining to this this position.External: Weekly contact with research collaborators from other institutions to share data or information produced in the lab. Monthly contact with vendors to exchange information.Students: Daily contact with undergraduate and graduate students working in the lab or a collaborator's lab to share ideas, information, skills, and lab priorities. Daily contact with student employees to help with experiments.SUPERVISORY RESPONSIBILITYThis position has no direct supervision of staff employees.QUALIFICATIONSEducation/Experience: Bachelor of Science