Description

　　The laboratory within the Department of Human Genetics works in the fields of epigenomics and neurodevelopmental disorders.

　　Projects in the lab use epigenomic profiling of primary tissues and disease modeling using in vitro cell culture models.

　　The incumbent will be primarily responsible for training and supervising junior lab members (e.g. SRAs) to perform experimental

　　works. The incumbent will supervise two SRAs to perform single cell epigenomic experiments using the Beckman Biomek automation

　　platform, performing key quality controls of the data. The incumbent will additionally supervise another SRA to generate spatial

　　transcriptomics data using the Nanostring CosMx spatial molecular imager. The incumbent will further supervise another SRA to

　　perform experiments to develop the photonic-indexing-sequencing approach. The incumbent will oversee the data generation for

　　two NIH U01 projects awarded to the PI's group and involves multiple collaborating labs at UCLA, in additional to external

　　collaborators at other universities.

　　Under the general supervision of the Principal Investigator, the incumbent will be responsible for undertaking research projects in

　　collaboration with the Principal Investigator (PI) making independent contributions to research and experiments regarding the roles

　　of gene regulation in brain development and psychiatric disorders using epigenomic approaches. The incumbent will perform

　　epigenomic profiling experiments, making significant independent contributions in the design, troubleshooting, modification, and

　　analysis of experiments and protocols.

　　The incumbent will be responsible for implementing and performing a variety of new and ongoing experimental protocols, making

　　significant and innovative contributions to the laboratory pertaining to complex experimental procedures of single-cell epigenomic

　　profiling. The incumbent may provide training and direction and work cooperatively with other lab members, including doctoral and student lab assistants. The incumbent will independently write and publish manuscripts in journals, attend and present research results at laboratory meetings and academic conferences, and draft grant proposals.

　　PAY RANGE: $ 24.28 - 48.04Qualifications

　　REQUIRED:

　　Experience in managing general responsibilities, such as ordering, maintenance of freezer

　　programs and laboratory equipments; teaching students and other UCLA personnel basic

　　molecular genetic methods, next-generation sequence analysis and new procedures.

　　Training and experience in the library making and data analysis of next-generation

　　sequencing data, including RNA sequencing and exome sequencing as well as in multiple

　　genotyping techniques as well as molecular, biochemical and cellular research.

　　Proficiency in the use of different computer clusters, servers, systems and languages,

　　including as R, Python, SAS, SPSS, PLINK, Mendel, WGCNA, Sequencer, Unix-based programs,

　　and PSQ96MA.

　　Excellent written and verbal communication skills.

　　Ability to perform independently organize and prioritize jobs (for self and assigned lab staff),

　　manage time, and meet deadlines.

　　Demonstrated ability to perform complex bioinformatics searches and comparisons in

　　genome databases such as NCBI (National center of biotechnology Information), UCSC

　　Genome Browser, HaploReg, BIOGPS, Genevar, PolyPhen, Sift.

　　Knowledge in lab safety including waste management.

　　Ability in designing study strategies.

　　Demonstrated ability in designing experiments and/or laboratory techniques.

　　Demonstrated ability to establish new laboratory procedures and protocols successfully.

　　Demonstrated skills in analyzing information, problems, and procedures to define the

　　problem and objective, identify relevant factors, formulate logical solution and follow up on

　　problem resolution.

　　Ability to perform molecular cloning and transformation of E. coli and eukaryotic cell lines.

　　Preferred

　　Ability to perform molecular biology and genetics procedures, i.e., SNP and microsatellite

　　genotyping, microarray analyses (using both SNP genotyping and expression arrays), resequencing,

　　PCR, RT-PCR, qRT-PCR, RNAse Protection Assay, genomic DNA analysis,

　　preparation of recombinant plasmids, transient and stable transfections, in vitro

　　transcription, mutagenesis and general recombinant DNA methods. Ability to analyze nextgeneration

　　sequence data derived from DNA and RNA sequencing.

　　PREFERRED:

　　Ability to maintain excellent professional working relationships with all levels of individuals.Ability to perform molecular cloning and transformation of E. coli and eukaryotic cell lines.

　　UCLA Health welcomes all individuals, without regard to race, sex, sexual orientation, gender identity, religion, national origin or disabilities, and we proudly look to each person’s unique achievements and experiences to further set us apart.