Reference #: 1007434SHIFT:Day (United States of America)

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　　A Brief OverviewThis Research Technician II will work in the laboratory of Karla Leavens, M.D., Ph.D. in the Division of Endocrinology and Diabetes at the Children's Hospital of Philadelphia. The position involves working alongside a physician scientist investigating diabetes pathogenesis with significant translational implications. Our research focuses on using human cell models to study diabetes, including use of pancreatic beta cells differentiated from human pluripotent stem cells and cadaveric human islets. We are part of the Research Institute at CHOP, which fosters a multidisciplinary approach among researchers and scientists working to discover new gene and cell therapies in search of cures for debilitating and life-threatening disorders.

　　Laboratory experience and familiarity with basic molecular laboratory techniques including PCR, gel electrophoresis, tissue culture, and DNA/RNA isolation are required. The technician will be trained in stem cell culture and differentiation, flow cytometry, molecular cloning, including CRISPR/Cas9, and other advanced cellular and molecular biology techniques as needed. Prior experience in any of these specialties is a plus. Specific duties will also include the maintenance and differentiation of human stem cell lines, performing experiments on these cells as directed, and maintaining appropriate laboratory notebooks, records, and protocols. The candidate will focus on pancreatic endocrine cell development to develop in vitro models of diabetes.

　　What you will doPerforms routine tests / experiments under the direction of principal investigator, supervisor or other designeePerforms research procedures on experimental samples as directedRecords and checks experiments/ results for completeness and accuracyPerforms calculations and maintains appropriate log and lab notebooksPerforms tasks that require familiarity with fundamental techniques and lab equipmentMay assist with planning specific research protocols and proceduresMaintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)Assists in care and maintenance of lab equipmentMay coordinate schedules of tests and proceduresAttends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniquesRecords results, organizes data and maintains lab notebook in an organized mannerCompiles and helps analyze data, prepares charts, graphs, and results of studies as directedConducts literary searches as directedEducation QualificationsHigh School Diploma / GED RequiredAssociate's Degree PreferredBachelor's Degree PreferredExperience QualificationsAt least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory exp rience RequiredAt least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience PreferredSkills and AbilitiesIntermediate understanding of lab procedures, protocols, and equipment.Basic exposure to lab specific experiments / techniques and scientific analysisExcellent verbal and written communications skillsExcellent organizational skillsSolid critical thinking / problem-solving skillsSolid analytical skills

　　To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine.

　　Employees may request exemptions for valid religious and medical reasons. Start dates may be delayed until candidates are immunized or exemption requests are reviewed.

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