Med-Medicine - Pennsylvania-Pittsburgh - (23009354) The Division of Pulmonary, Allergy, Critical Care and Sleep Medicine is looking for a Senior Research Technician. The position requires performance of specialized techniques to understand various aspects of lung disease with focus on asthma and acute respiratory distress syndrome (ARDS). Experiments will involve:1) Processing of human samples from healthy subjects, asthma, ARDS, and sepsis patients for immune cell enrichment for immediate use and cryopreservation. The samples will include blood and lung lavage. Experiments will be performed with enriched cells.2) Implementation of a new technique of high dimensional flow cytometry for analysis of human and mouse immune cells.Job SummaryPerforms a variety of independent and complex laboratory work and experiments. Analyzes and interprets data, compiles results, and prepares reports. Maintains inventory, ordering, and logs. Writes manuscripts and grant proposals and prepares data visualizations.Essential Functions 1. Processing of human samples from lungs and peripheral blood by density gradient centrifugation and further processing for cryopreservation using specialized protocols. 2. Breeding of genetically modified mice and genotyping. 3. Setting up mouse models of lung diseases and analysis at the completion of the experiment. 4. Analysis of human and mouse samples by qRT-PCR, ELISA and related techniques.5. Acquisition of a new sophisticated skill-set of multiparameter, high dimensional flow cytometry for immune cell profiling. 6. Analysis of tissue by histology, immunological, and microscopic techniques. 7. Equipment and reagent maintenance and ordering of lab supplies. 8. Ensuring compliance with University and Federal Government rules for proper functioning of the lab. 9. Supervision of lower-level technicians, undergrad students, clinical (pulmonary) fellows, and other trainees in the laboratory. Development of training programs to facilitate the acquisition of laboratory skills by trainees and new hires.Physical Effort -          Must be able to stand and/or sit for up to 8 hours. The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.The University of Pittsburgh requires all Pitt constituents (employees and students) on all campuses to be vaccinated against COVID-19 or have an approved exemption. Visit hr.pitt.edu/contact-ohr to learn more. Assignment Category Full-time regularJob Classification Staff.Laboratory Research Technician IVJob Family ResearchJob Sub-Family LaboratoryCampus PittsburghMinimum Education Level Required Bachelor's DegreeMinimum Years of Experience Required 3Will this position accept substitution in lieu of education or experience? Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.Work Schedule Monday -- Friday, 8am-4:30pmWork Arrangement On-Campus: Teams that work on campus, in an office, or in a lab.Hiring Range TBD Based Upon QualificationsRelocation Offered NoVisa Sponsorship Provided YesBackground Check For position finalists, employment with the University will require successful completion of a background checkChild Protection Clearances Not ApplicableRequired Documents ResumeOptional Documents Cover Letter