Job ID358004 LocationTwin Cities Job FamilyResearch-Researchers Full/Part TimeFull-Time Regular/TemporaryRegular Job Code8352R2 Employee ClassCivil Service About the Job Position Summary: We seek a researcher to join a highly collaborative research group in the University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics. The researcher will assist experienced scientists in carrying out large-scale molecular biology workflows including cloning, mutagenesis, and antibody engineering developing cancer therapeutics. The researcher will keep clear and extensive records of scientific work and perform basic lab maintenance including reagent stock management and ordering. The researcher will participate in weekly laboratory meetings to discuss ongoing research with the research team. Essential Functions: 30% Molecular Biology and Cell Culture including: DNA & RNA isolation and sequencing; Nucleic acid gel electrophoresis; PCR of diverse DNA templates; PCR primer design and optimization; PCR reaction optimization; Plasmid design including design of expression plasmids for recombinant protein expression in bacteria, insect, and mammalian cells; Construction of plasmids of varying size using a variety of methods including conventional restriction enzyme ligation approaches, Gibson Assembly and/or Golden Gate Cloning: Plasmid mutagenesis; Generation of DNA libraries; Culture and maintenance of mammalian cell lines. 30% Protein biochemical and biophysical assays. Elisa, Western blotting, optical spectroscopy, protein thermal stability, protein-protein interaction characterization. 30% Recombinant Protein Expression and Purification and Crystallography Protein expression in Bacteria and Mammalian cells. Protein purification using affinity tags. Protein purification using chromatography systems. 10% General Laboratory Maintenance Inventory and organize laboratory materials, Maintain general working order of laboratory, Assist in ordering materials for laboratory work. Qualifications All required qualifications must be included in the application materials Required Qualifications: BA/BS and 1 year of related experience or equivalent combination of education and work experience to equal five (5) years in any of the following areas: Microbiology, Chemistry, Biochemistry, Biology, Pharmacology, or related field. Preferred Qualifications: BA/BS and 1 year of related experience or equivalent combination of education and work experience to equal five (5) years in Molecular Biology, Mammalian Cell culture, or Protein expression and purification experience. About the Department BMBB: The department of Biochemistry, Molecular Biology and Biophysics is committed to understanding the molecular mechanisms of metabolic diseases and cancer; developing novel strategies in biocatalysis and biotechnology; and advancing knowledge through structural biology and molecular biophysics. We organize ourselves into research divisions with emphases on Chemical and Structural Biology, Metabolic and Systems Biology, Molecular Biology and Synthetic Biology and Biotechnology. We are housed in new buildings with state-of-the-art research and support facilities. Benefits Working at the University At the University of Minnesota, you'll find a flexible work environment and supportive colleagues who are interested in lifelong learning. We prioritize work-life balance, allowing you to invest in the future of your career and in your life outside of work. The University also offers a comprehensive benefits package that includes: Competitive wages, paid holidays, and generous time off Continuous learning opportunities through professional training and degree-seeking programs supported by the Low-cost medical, dental, and pharmacy plans Healthcare and dependent care flexible spending accounts University HSA contributions Disability and employer-paid life insurance Employee wellbeing program Excellent retirement plans with employer contribution Public Serv ce Loan Forgiveness (PSLF) Financial counseling services Employee Assistance Program with eight sessions of counseling at no cost with free or reduced rates in the Twin Cities metro area Please visit the for more information regarding benefits. How To Apply Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume. Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section. To request an accommodation during the application process, please e-mail [email protected] or call (612) 624-UOHR (8647). Diversity The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: Employment Requirements Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment. About the U of M The University of Minnesota, Twin Cities (UMTC) The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations. At the University of Minnesota, we are proud to be recognized by the Star Tribune as a Top Workplace for 2021, as well as by Forbes as Best Employers for Women and one of America's Best Employers (2015, 2018, 2019, 2023), Best Employer for Diversity (2019, 2020), Best Employer for New Grads (2018, 2019), and Best Employer by State (2019, 2022). The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.