Research Technician IEmployment TypeRegular Full-TimeRequisition number1894DepartmentRay LabLocation : CitySeattleOverviewThe Ray Lab at Benaroya Research Institute (BRI) in Seattle, WA is seeking a highly-motivated Research Technician. The Ray lab intersects immunology and genetics to define genetic mechanisms that drive risk for autoimmune diseases such as lupus, multiple sclerosis, type 1 diabetes, and rheumatoid arthritis, among others. The lab performs functional genomic approaches in immune cells to identify genetic variants, genes, and cis-regulatory regions that likely promote disease risk and clarifies their mechanisms using human and mouse cellular and molecular immunological approaches. Dr. Ray has affiliate appointments at the University of Washington Departments of Immunology and Genome Sciences.ResponsibilitiesWorking with mice, including genotyping, general colony maintenance, immunizations, viral infections and tissue harvest for cellular isolation and analysis.Isolation and culture of primary cells, as well as maintenance of common adherent and non-adherent cell line cultures.Performing chemical, physical, or biological assays following written protocols. Protocols include but are not limited to:Antibody staining of cells and flow cytometry.DNA isolation and PCR.Lentivirus preparation and infection of cells using BSL2 level safety techniques.Preparing fractionated samples from blood using sterile technique.Operating a variety of laboratory instruments such as spectrometers, electrophoresis equipment, PCR machines, measuring instruments and devices.Perform computational analysis of genomic sequencing data.Maintain laboratory records by recording detailed data, including photography and computer input, in a literate and thorough manner.Prepare progress reports including gathering, organizing, and interpreting data and preparing data for presentations and manuscripts.Conduct literature searches and read literature applicable to research area.Conduct lab inventories and maintain adequate quantities of reagents and supplies for the lab, placing orders as necessary.Some weekend work will be required during long-term animal studies or maintenance of sensitive cell cultures.QualificationsBA/BS in a scientific field and basic laboratory skills required.Knowledge of basic molecular biology techniques.Must have strong mathematical, computer, data analysis, and statistical analysis skills.Proven good verbal and writing skills as well as have the ability to work both independently and in a team environment.Compensation$22.46 to $31.89 per hr.BenefitsMedical, dental, vision hardware insuranceFlexible spending accounts: health care, dependent care, commuterShort and long-term disability and life and AD&D insurance403(b) retirement plan with matching funds after one year of employmentPTO: 13 holidays, three weeks of vacation and two weeks of sick leave per yearEmployee assistance programEducational assistance ProgramSubsidized bus passSubsidized YMCA membershipVoluntary benefits, e.g., pet insurance, accident, critical illness, identify theftSee benaroyaresearch.org for benefit detailsAbout UsBenaroya Research Institute (BRI) has a bold mission: To predict, prevent, reverse and cure immune system diseases, from autoimmune disease to cancer to COVID-19. We examine the immune system in both health and disease to understand how disorders start and how to rebalance the immune system back to health. Equipped with innovative tools and robust biorepositories, our team chips away at the biggest mysteries behind these conditions to work toward our