The Pathology Department is seeking a proficient Research Specialist II with a sophisticated understanding of techniques and a solid foundation in the underlying science, gained from experience. Experience in animal breeding, and maintenance preferred. Will perform animal surgeries and collect/process tissue samples. Will perform experiments including cell culture, western blotting, and immunohistochemistry with minimal supervision. Will manage the daily operation of the lab, including reagent preparation, ordering, inventory, and supervision of equipment. Will provide training as needed to individuals who may be assigned to the laboratory on a regular or temporary basis. The research specialist must be open to learning new techniques, developing and recording new protocols, and communicating regularly and effectively with the principal investigator. This individual should have a strong desire to become part of a dynamic and inclusive team.

　　Specific Duties & Responsibilities

　　Maintain mouse colonies, performing particular breeding schemes, genotyping, and weaning. Perform survival surgeries, collect and process tissues, and maintain detailed databases.

　　Maintain multiple cell lines, associated databases, and routine testing of cells.

　　Perform daily experiments including immunohistochemistry, western blotting, and cell treatments.

　　Learn additional methods as needed including, but not limited to; ELISA, flow cytometry, laser capture microdissection, and qRT-PCR.

　　Scanning of histological slides, organization of slides and images, as well as generation and management of associated databases

　　Maintain lab inventories, order supplies as needed, and organize general lab areas.

　　Maintain databases and organize clinical and animal specimens and reagent resources.

　　Create and implement laboratory standard operating procedures of all aspects of sample handling and analysis.

　　Record outcomes/results of all experiments by writing.

　　Report experimental procedures, results, and problems to principal investigator in a timely fashion.

　　Attend weekly lab meetings to provide general updates and receive updates from lab members.

　　Exchange routine information in an appropriate manner.

　　Regular interaction with students, vendors, post-doctoral fellows, faculty members, and other clinical fellows.

　　Train students, staff members, and postdoctoral fellows with established protocols.

　　Assist the principal investigator to coordinate the responsibilities for different projects and manage the laboratory operation.

　　Responsible for preventative maintenance of equipment and laboratory operation procedures.

　　Maintains laboratory standards and university policies.

　　Perform miscellaneous related duties as assigned.

　　Equipment, Machine, or Tool Requirements

　　Computer and Laboratory equipment maintaining and operation lab such as: Cell culture systems, whole slide scanner, spectrophotometer, centrifuges, freezers, vacuum, chemiluminescence/fluorescence imager etc.Special Knowledge, Skills, & Abilities

　　Reasonable computer skills. Have strong communication skills.

　　Uses various software applications, such as Microsoft Excel, GraphPad prism, and image analysis software.

　　Adaptable to a fast-pace, dynamic environment.

　　Specific Physical Requirements

　　While performing the duties of this job, the employee may remain in a stationary position (sitting or standing) up to 70% of the time.

　　Occasionally required to traverse throughout the laboratory or to retrieve equipment, supplies, or specimens from other locations on campus.

　　The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes, cutting instruments, glass tubes, dishes, and/or slides.

　　Constantly repositions self to perform duties at laboratory workstations, gathering specimens, and loading equipment.

　　Prolonged vision requirements including viewing computer screen, paper reports/documents, charts and results.

　　Visual acuity is required to distinguish fine gradation of color or structure and closely examine specimens (including via a microscope).

　　Laboratory Environment

　　Exposure to toxins and infectious agents exist, but potential for personal injury or harm is minimized if established safety and health precautions are followed.

　　Must refer to and are expected to comply with procedure manuals, follow proper laboratory protocols and safety policy/procedures, and be familiar with material data safety sheets in assigned work areas.

　　Able to read and understand all Health, Safety and Environment (HSE) guidelines applicable to assigned work area.

　　Working in laboratory where there may be discomforts due to odors, noise, temperature fluctuations, and working around lab equipment.

　　Research areas may include animal, human, human products, DNA, radiation protocols, and other bio-hazardous materials.

　　Use of personal protective equipment may be advised or required.

　　May transport equipment and supplies usually less than 40 pounds from one area to another (in the laboratory or on campus (specify).

　　May be required to lift and/or move up to 50 pounds with proper training, or precautions/lifting aides (example: supply boxes or Formalin cubes).

　　In some areas workspace is limited.

　　Staff may be required to complete annual competency review, and must insure compliance with Johns Hopkins Policy, Laboratory Policy & Procedure, Health, Safety & Environment regulations, and all applicable privacy & confidentiality laws/practices. Rotating on-call, weekend, or pager duties are required.

　　Many department laboratories operate 24/7 with schedules on day, evening, or night shift.

　　Rotating schedules which may include weekend work and holiday assignment. (Overtime possible when authorized/needed to meet business and patient needs).

　　In the event of unexpected university closings including inclement weather or declared emergency, the employee in this position is required to report to work and/or remain at work.

　　Minimum Qualifications

　　Bachelor's Degree in Biology, Chemistry or related field.

　　Two years laboratory experience performing relevant techniques.

　　Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.

　　Preferred Qualifications

　　Previous experience with animal maintenance.

　　Master’s level training – in progress or complete.

　　Classified Title: Research Specialist II

　　Role/Level/Range: ACRP/03/MB

　　Starting Salary Range: Min $40,900 - Max $71,600 Annually ($56,641 targeted; Commensurate with experience)

　　Employee group: Full Time

　　Schedule: M-F, 8:00am-4:30pm (flexible)

　　Exempt Status: Exempt

　　Location: School of Medicine Campus

　　Department name: ​​​​​​​SOM Pat Kidney Urologic Pathology

　　Personnel area: School of Medicine

　　Total Rewards

　　The referenced salary range is based on Johns Hopkins University’s good faith belief at the time of posting. Actual compensation may vary based on factors such as geographic location, work experience, market conditions, education/training and skill level. Johns Hopkins offers a total rewards package that supports our employees' health, life, career and retirement. More information can be found here: https://hr.jhu.edu/benefits-worklife/

　　Please refer to the job description above to see which forms of equivalency are permitted for this position. If permitted, equivalencies will follow these guidelines:

　　JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

　　**Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

　　The successful candidate(s) for this position will be subject to a pre-employment background check. Johns Hopkins is committed to hiring individuals with a justice-involved background, consistent with applicable policies and current practice. A prior criminal history does not automatically preclude candidates from employment at Johns Hopkins University. In accordance with applicable law, the university will review, on an individual basis, the date of a candidate’s conviction, the nature of the conviction and how the conviction relates to an essential job-related qualification or function.

　　The Johns Hopkins University values diversity, equity and inclusion and advances these through our key strategic framework, the JHU Roadmap on Diversity and Inclusion (https://diversity.jhu.edu/roadmap-on-diversity-and-inclusion/) .

　　Accommodation Information

　　If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the Talent Acquisition Office at [email protected] . For TTY users, call via Maryland Relay or dial 711. For more information about workplace accommodations or accessibility at Johns Hopkins University, please visit accessibility.jhu.edu .

　　Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. The COVID-19 vaccine does not apply to positions located in the State of Florida. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit https://www.hopkinsmedicine.org/coronavirus/covid-19-vaccine/ and all other JHU applicants should visit https://covidinfo.jhu.edu/health-safety/covid-vaccination-information/ .

　　The following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.

　　The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

　　Note: Job Postings are updated daily and remain online until filled.

　　Equal Opportunity Employer:

　　Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.